Composite

Part:BBa_K2557013:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-miniCMV-PhiC31


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 392
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 325
    Illegal BamHI site found at 1243
    Illegal BamHI site found at 1329
    Illegal BamHI site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 392
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 392
    Illegal NgoMIV site found at 410
    Illegal NgoMIV site found at 639
    Illegal NgoMIV site found at 681
    Illegal AgeI site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 935
    Illegal BsaI.rc site found at 1338
    Illegal BsaI.rc site found at 1691


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of PhiC31 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References